The important core structure of human milk oligosaccharides (HMOs) is composed of galactose, N-acetylglucosamine, and glucose. It has prebiotic effects, immune regulatory effects, anti-inflammatory effects, intestinal cell response regulatory effects, antibacterial activity, and antiviral activity. In the United States and the European Union, LNT has been approved for use in various foods and beverages such as infant formula, beverages, and dairy products. In addition, the EU can also apply LNT to dietary supplements, formula foods for special medical purposes, and milk based beverages or similar products intended solely for young children.

One of the most important neutral core HMOs, it has been certified as safe by the US FDA (GRAS) and is the second HMO approved by the National Health Commission in China to be added to infant formula after 2 '- fucosyllactose (2' - FL). LNnT has been approved for use in various foods and beverages such as infant formula, beverages, dairy products, etc. in the United States and the European Union. In addition, the EU can also apply LNnT to dietary supplements (for young children and the general population), formula foods for special medical purposes, and milk based ingredients or similar products intended solely for young children. In China, it has been approved for use in infant formula, formulated milk powder (limited to children's milk powder only), and special medical use infant formula.